Coding

Part:BBa_K4467006

Designed by: Eloise Paine   Group: iGEM22_UNSW_Australia   (2022-10-10)


SERK3B

Properties and Biology

SERK3B is a brassinosteroid insensitive 1-Associated Kinase 1.This plant receptor-like protein has roles in response to pathogen-associated molecular pattern (PAMP) molecules, triggering the PAMP-regulated immunity response (Dagvadorj et. al., 2017).

Usage

As part of the 2022 UNSW_Australia project to design a peptide based inhibitor for Puccinia sp., we used SCR1 as a potential target. This protein was expressed in NEB TURBO E.coli cells.

References

Dagvadorj, B., Ozketen, A. C., Andac, A., Duggan, C., Bozkurt, T. O. and Akkaya, M. S."A Puccinia striiformis f. sp. tritici secreted protein activates plant immunity at the cell surface", Scientific Reports, 7(1). doi: 10.1038/s41598-017-01100-z. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 204
    Illegal EcoRI site found at 666
    Illegal PstI site found at 283
    Illegal PstI site found at 850
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 204
    Illegal EcoRI site found at 666
    Illegal PstI site found at 283
    Illegal PstI site found at 850
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 204
    Illegal EcoRI site found at 666
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 204
    Illegal EcoRI site found at 666
    Illegal PstI site found at 283
    Illegal PstI site found at 850
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 204
    Illegal EcoRI site found at 666
    Illegal PstI site found at 283
    Illegal PstI site found at 850
    Illegal AgeI site found at 543
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 712


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Parameters
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