Coding
Part:BBa_K4467006
Designed by: Eloise Paine Group: iGEM22_UNSW_Australia (2022-10-10)
SERK3B
Properties and Biology
SERK3B is a brassinosteroid insensitive 1-Associated Kinase 1.This plant receptor-like protein has roles in response to pathogen-associated molecular pattern (PAMP) molecules, triggering the PAMP-regulated immunity response (Dagvadorj et. al., 2017).
Usage
As part of the 2022 UNSW_Australia project to design a peptide based inhibitor for Puccinia sp., we used SCR1 as a potential target. This protein was expressed in NEB TURBO E.coli cells.
References
Dagvadorj, B., Ozketen, A. C., Andac, A., Duggan, C., Bozkurt, T. O. and Akkaya, M. S."A Puccinia striiformis f. sp. tritici secreted protein activates plant immunity at the cell surface", Scientific Reports, 7(1). doi: 10.1038/s41598-017-01100-z. Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 204
Illegal EcoRI site found at 666
Illegal PstI site found at 283
Illegal PstI site found at 850 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 204
Illegal EcoRI site found at 666
Illegal PstI site found at 283
Illegal PstI site found at 850 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 204
Illegal EcoRI site found at 666 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 204
Illegal EcoRI site found at 666
Illegal PstI site found at 283
Illegal PstI site found at 850 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 204
Illegal EcoRI site found at 666
Illegal PstI site found at 283
Illegal PstI site found at 850
Illegal AgeI site found at 543 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 712
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Categories
Parameters
None |